Paracrine Modification of Interrenal Tissue by Reproductive Hormones in Rainbow Trout (Oncorhynchus mykiss)
Alyahya, Saherah E.
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Reproductive systems are known to be very sensitive to stress (Lovejoy et al., 2011). An active stress response is often associated with negative influences on all reproductive system levels. However, the mechanisms of action are far from clear (Gennotte et al., 2012; Alsop et al., 2009). The influence was thought to be a consequence of the interactions between the hypothalamic-pituitary-gonadal (HPG) and the Hypothalamic-Pituitary-Adrenal (HPA)/ Hypothalamic-Pituitary-Interrenal (HPI) axes (Alsop, 2011). Current information suggests that the reproductive system is partially regulated via paracrine transforming growth factor β (TGF-β) signals in ovarian development and steroidogenesis in mammals and birds, but few studies have been done on fish (Lankford & Weber, 2010). In this study we investigate the interrenal role of TGF-β members (Activin A and Bone morphogenetic protein 7 (BMP7)) and Bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) via a tissue culture preparation of rainbow trout interrenal cells stimulated with adrenocorticotropic hormone and estradiol. Target transcripts were measured using quantitative real-time PCR. Expression of all three transcripts was confirmed, but due to a technical error that prevented analysis of all samples the results on modification are only speculative. In conclusions, we developed a head kidney tissue culture technique and established the expressions of TGFβ superfamily in the Interrenal of fishes. Furthermore, these data suggest interrenal Activin A and BMP7 might serve to modify oocyte maturation during times of stress through cross-talk between the stress and reproductive axes that involves the TGFβ superfamily.