Identifying the Flavonoid 3', 5'-hydroxylase Gene in Dianella, a Relative of Daylily (Hemerocallis)
Alatawi, Nouf M.
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Flavonoids represent a large class of molecules of which anthocyanins are the essential pigments of flowers. Major classes of anthocyanins are controlled by cytochrome P450 enzymes, which alter anthocyanins to become red to blue to purple pigments. One of the cytochrome P450 enzymes, flavonoid 3’,5’ hydroxylase, is important for the synthesis of the 3’, 5’-hydroxylated anthocyanins responsible for blue pigment in plants. Daylilies (Hemerocallis) do not produce blue flowers or fruit, but Dianella, a relative of daylily does. The long-term goal of the project is to identify the effects of the gene coding for flavonoid 3’, 5’ hydroxylase (F3’5’H) on daylily versus Dianella flower color. The first step of this investigation is to sequence DNA fragments of the F5'3'H gene. The F3’5’H gene was amplified from Glycine max (positive control) DNA using the designed primer pair 3.